Biology Application Notes
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Reference |
Type |
Title |
Brief Summary |
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In vivo Raman Measurements of Human Skin |
Raman measurements are carried out using an inverted microscope to probe the skin on the index fingertip. Crucial parameters such as confocality, excitation wavelength are discussed. The Raman spectra provide information on lipids, proteins, water concentration at several depths of the skin. | |
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SERS analysis of single living lymphocytes |
Surface enhanced Raman spectroscopy (SERS) and multivariate data analysis has been used to study the distribution of Rhodamine 6G (acting as a model chemotherapeutic drug) within single living lymphocyte cells. | |
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Raman analysis of single bacteria cells |
Confocal Raman analysis of single bacteria cells provides a characteristic biochemical fingerprint, and multivariate data analysis of the complex spectra allows the speciation, growth stage and isotope uptake of the cells to probed | |
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Raman mapping of wheat grain kernels |
Raman analysis of thinly sectioned wheat kernels has identified the main constituents, and their distribution has been mapped. Close analysis of the Amide I band allows the grain hardness and protein secondary structure to be correlated. | |
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SERS for Intracellular Imaging |
The localization and interaction of anticancer agent mitoxantrone has been investigated at the intracellular level using confocal surface enhanced Raman spectroscopy (SERS). Comparison of in vivo and in vitro drug analysis clearly identifies binding of the drug to DNA within the cell, and shows the power of Raman in understanding drug-cell interactions more fully. | |
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Insights into thrombosis mechanisms using high resolution SERS |
Surface enhanced Raman spectroscopy (SERS) has been used to provide an insight into the activation mechanisms of a glycoprotein which plays an important role in thrombosis. Different techniques for generating the gold SERS substrate have also been investigated, to identify the best method of obtaining reproducible, high enhancement SERS. | |
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Raman investigation of microorganisms on a single cell level |
Identification of contaminating microbes is an important public safety issue in many fields. Previous research has shown the potential for Raman spectroscopy in combination with chemometric methods to investigate bulk samples as well as a single bacterium and yeast cell for classification on the species and strain level. |
